LCMV Plaque Assay Medium

Dillon Corvino

Medium used to overlay infected Vero cell monolayers during LCMV plaque assays.
Published

September 18, 2026

Protocol ID: BUF-LCMV-PLA Version: v1.1 Author: Dillon Corvino Date: September 18, 2026

Purpose

Medium used to overlay infected Vero cell monolayers during LCMV plaque assays.

Linked protocols

  • MUS-LCMV-PLA

Working buffer composition

Final volume: 250 mL

Component Amount
2x Eagle MEM without phenol red 250 mL
200 mM L-glutamine 5 mL
Heat-inactivated FBS (10%) 25 mL
Pen/Strep (10,000 U/mL penicillin G, 10,000 ug/mL streptomycin) 5 mL
Fungizone (amphotericin B 250 ug/mL) 2.5 mL
Note

Fungizone is optional but recommended to prevent fungal contamination.

Preparation

A. Preparation of stock (if applicable)

Not applicable — prepared directly as the working buffer.

B. Preparation of working buffer

  1. Combine all components in a sterile bottle.
  2. Mix thoroughly by gentle inversion.

Storage and stability

  • Store at 4°C for up to 4 weeks.

Reagent details

Component Supplier Cat# Notes
2x Eagle MEM without phenol red [TBD] [TBD] Base medium
200 mM L-glutamine [TBD] [TBD]
FBS [TBD] [TBD] Heat-inactivated
Pen/Strep [TBD] [TBD] 10,000 U/mL penicillin G, 10,000 ug/mL streptomycin
Fungizone (amphotericin B) [TBD] [TBD] 250 ug/mL; optional but recommended to prevent fungal contamination

Safety (brief)

  • FBS is of animal origin and should be treated as a potential biohazard.
  • This medium overlays LCMV-infected Vero cell monolayers — handle infected plates and this medium according to institutional biosafety protocols for virus-containing material.

Version history

Version Date Author Changes
v1.0 2025-12-01 Dillon Corvino Initial Quarto buffer document
v1.1 2026-09-18 Dillon Corvino Migrated to shared summary:/helper architecture (buffer/protocol redesign); added Storage/Safety/Reagent-details sections.